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Usbio/357883 PAH (Phenylalanine Hydroxylase) BioAssay™ ELISA Kit (Human)/357883/48Tests
Usbio/357883 PAH (Phenylalanine Hydroxylase) BioAssay™ ELISA Kit (Human)/357883/48Tests
商品编号：
357883
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PAH (Phenylalanine Hydroxylase) BioAssay™ ELISA Kit is a quantitative sandwich assay for the detection of PAH in human serum, plasma, tissue homogenates and other biological fluids
Detection Range
78.125-5000pg/ml
Sensitivity
<46.875pg/ml
Precision: t|Intra-Assay CV: <8%|Inter-Assay CV: <10%
Assay Principle
This ELISA kit employs the sandwich enzyme-linked immunoassay technique, utilizing a microtiter plate pre-coated with an antibody specific to PAH. Standards and samples are added to the appropriate wells, then incubated. Aspirate. Do not wash the plate! The Antibody (Biotin) is added to all the wells. The plate is incubated and then washed. Streptavidin (HRP) is added to each microplate well and incubated then washed. Then the TMB substrate solution is added and incubated. After the TMB substrate solution is added, only those wells that contain PAH, the antibody (Biotin) and Streptavidin (HRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAH in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
*357883A: Microtiter Strips, 1x96 wells (8x12 wells).*357883B: Standard, 2x1 vial357883C: Sample/Standard Dilution Buffer, 1x20ml357883D: Antibody (Biotin) (Concentrated), 1x120ul357883E: Antibody Dilution Buffer, 1x10ml357883F: Streptavidin (HRP) (SABC), 1x120ul357883G: SABC Dilution Buffer, 1x10ml357883H: TMB Substrate, 1x10ml357883J: Stop Solution, 1x10ml357883K: Wash Buffer, 25X, 1x30ml
Storage and Stability
Store unopened *357883A at 4ºC; store at -20ºC once opened. Store unopened *357883B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary
1. Wash plate 2 times before adding standards and samples to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C. Aspirate. Do not wash plate!3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37°C. 4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
品牌介绍
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