当前位置：
首页
>
产品中心 >
Sandwich_method_ELISA >
Usbio/219609 Cathepsin D, BioAssay™ ELISA Kit (Mouse)/219609/100Tests
Usbio/219609 Cathepsin D, BioAssay™ ELISA Kit (Mouse)/219609/100Tests
商品编号：
219609
品牌：
USbiological
市场价：
¥21040.00
美元价：
8942.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
This mouse Cathepsin D BioAssay™ ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology.
Sample Type
Serum and plasma (heparin, EDTA), cell culture supernatants.
Intended Use
For use in quantitative protein detection, establishing normal range etc.
Sensitivity
<10pg/ml
Range
156-10,000pg/ml
Specificity
Recognizes natural and recombinant mouse Cathepsin D. This ELISA kit shows no cross-reactivity with other relevant proteins.
Test Principle
This assay employs a monoclonal antibody form rat specific for Cathepsin D coated on a 96-well plate. Standards (NSO) and test samples are added to the wells and Cathepsin D present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin D captured onto the plate.
Kit Components
Microplate coated with anti-mouse Ab against Cathepsin D, 1x96 wellsLyophilized recombinant mouse Cathepsin D standard (10ng/vial), 2x vials tBiotinylated anti-mouse Cathepsin D antibody, 1x130ul tAvidin-Biotin-Peroxidase Complex (ABC), 1x130ul tSample diluent buffer, 1x30ml tAntibody diluent buffer, 1x12ml tABC diluent buffer, 1x12ml tTMB color developing agent (Colorless), 1x10ml tTMB stop solution, 1x10ml t
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！
联络我们
