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Usbio/353009 Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9), BioAssay™ ELISA Kit (Mouse)/353009/96 Tests
Usbio/353009 Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9), BioAssay™ ELISA Kit (Mouse)/353009/96 Tests
商品编号：
353009
品牌：
USbiological
市场价：
¥14980.00
美元价：
6366.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Proprotein convertase subtilisin/kexin type 9, also known as PCSK9, is an enzyme that in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. By genomic sequence analysis, the PCSK9 gene was mapped to chromosome 1p32. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A(EGF-A) domain of the low-density lipoprotein receptor(LDLR), inducing LDLR degradation. Reduced LDLR levels result in decreased metabolism of low-density lipoproteins(LDL), which could lead to hypercholesterolemia. PCSK9 may also have a role in the differentiation of cortical neurons.
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Intended Use
For quantitative detection of mouse PCSK9 in cell culture supernates, serum and plasma (heparin, EDTA).
Sensitivity
<10pg/ml
Range
62.5-4000pg/ml
Specificity
Natural and recombinant mouse PCSK9
Test Principle
This mouse PCSK9 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PCSK9 has been precoated onto 96-well plates. Standards(NSO) from murine sarcoma basement membrane) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PCSK9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PCSK9 amount of sample captured in plate.
Kit Components
96-well plate precoated with mouse PCSK9 antibody, 1Lyophilized recombinant mouse PCSK9 standard, 4ng/tube×2Biotinylated mouse PCSK9 antibody, 130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100)Sample diluent buffer, 30mlAntibody diluent buffer, 12mlABC diluent buffer, 12mlTMB color developing agent, 10mlTMB stop solution, 10ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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