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Usbio/359610 VDR (Vitamin D3 Receptor) BioAssay™ ELISA Kit (Human)/359610/48Tests
Usbio/359610 VDR (Vitamin D3 Receptor) BioAssay™ ELISA Kit (Human)/359610/48Tests
商品编号：
359610
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
he Vitamin D3 Receptor (VDR) ELISA Kit (Human) is a sandwich assay for the quantitative detection of VDR in human serum, plasma and other biological fluids. Cell lysates and tissue homogenates, though not tested, may potentially be used as samples.
Detection Range
0.625-40ng/ml
Sensitivity
<0.375ng/ml
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to VDR. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDR, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VDR in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
*359610A: Microtiter Strips, 1x96 wells *359610B: Standard, 2x1vial359610C: Sample/Standard Dilution Buffer, 1x20ml359610D: Antibody (Biotin) Concentrate, 1x120ul359610E: Antibody Dilution Buffer, 1x10ml359610F: Streptavidin-HRP Conjugate (SABC), 1x120ul359610G: SABC Dilution Buffer, 1x10ml359610H: TMB Substrate, 1x10ml359610J: Stop Solution, 1x10ml359610K: Wash Buffer (25X), 1x30ml
Storage and Stability
Store unopened *359610A at 4ºC; store at -20ºC once opened. Store unopened *359610B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary
1. Wash plate 2 times before adding standards, samples and control (zero) to wells!2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate and wash 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate and wash 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
品牌介绍
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