The phage display technology is a powerful and well-established tool for the investigation of protein-protein interactions and for the generation of human antibodies for in vitro diagnostics and in vivo therapy (Koch et al. 2000). Generally several rounds of selection on an immobilized antigen are required to enrich and isolate the binding phage. The need to determine phage titers before and after each round to monitor the panning process still represents a major time and material consuming factor within this procedure. Usually, the phages are titered by infecting E. coli with serial dilutions of phage, plating the bacteria on agar plates and counting the colony (cfu) or plaque forming units (pfu) after over-night incubation (Koch et al., 2000). To determine the relative number of phage particles, however, immunotitration by Phage Titration ELISA offers a fast, sensitive and reproducible alternative for titration of M13 bacteriophage preparations.

Reagent kit, 12 x 8-well strips.

Incubation: |60/60 minutes

Standard Range: |5x10e6 -1x10e8 particles /ml

P3365-65A: Microtiter Strip. 1x96 wellsP3365-65B: Control (purified M13 particles), 2x1vialP3365-65C: Mab (HRP) 20X, 1x750ulP3365-65D: Wash Buffer, 20X, 1x20mlP3365-65E: TMB Substrate, 1x12mlP3365-65F: Stop Solution, H2SO4, 1x13ml

Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.