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Usbio/167568维生素B12（VB12，氰基钴胺）生物测定与贸易；ELISA试剂盒（人）/167568/96试验
Usbio/167568维生素B12（VB12，氰基钴胺）生物测定与贸易；ELISA试剂盒（人）/167568/96试验
商品编号：
167568
品牌：
USbiological
市场价：
¥18680.00
美元价：
7939.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Vitamin B12 ELISA Kit is an enzyme immunoassay (ELISA) for the quantitative detection of Vitamin B12 concentration in human serum. The Vitamin B12 ELISA test is for in vitro diagnostic use only.
Sensitivity: 70.13pg/ml
Test Principle
Delayed Competitive Enzyme Immunoassay (TYPE 9): The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody.
After a short incubation, the enzyme conjugate is added (This delayed addition permits and increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analogand the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation).|A simultaneous reaction between the biotin attachedto the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
Kit Components
167568A: Microtiter Plate 1x96 wells 167568B: Standard 0pg/ml 1x1ml167568C: Standard 100pg/ml 1x1ml167568D: Standard 200pg/ml 1x1ml167568E: Standard 400pg/ml 1x1ml167568F: Standard 1000pg/ml 1x1ml167568G: Standard 2000pg/ml 1x1ml167568H: Vitamin B12 (HRP): 1x7ml 167568J: Vitamin B12 (Biotin): 1x7ml167568K: Stop Solution (0.5M H2SO4):1x8ml167568L: Wash Solution, (PBS + Tween-20): 1x20ml 167568M: Substrate Solution (TMB, H2O2): 1x12ml167568N: Releasing Agent: 1x14ml 167568P: Stabilizing Agent: 1x700ul 167568Q: Neutralizing Buffer: 1x7ml
The calibrators can be expressed in molar concentrations (pM/L) by multiplying by 0.738. For example: 100pg/ml x 0.738 = 73.8pM/L.
Storage and Stability
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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