0.625-40ng/ml

< 0.228ng/ml

Intra-Assay: CV<10%Inter-Assay: CV<12%

The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VDR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VDR in the sample is then determined by comparing the O.D. of the sample to the standard curve.

*028899A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*028899B: Standard, 2x1vial 028899C: Standard Diluent, 1x20ml*028899D: Detection Reagent A, 1x120ul *028899E: Detection Reagent B, 1x120ul 028899F: Assay Diluent A, 1x12ml028899G: Assay Diluent B, 1x12ml028899H: TMB Substrate, 1x9ml028899K: Stop Solution, 1x6ml028899L: Wash Buffer, 30x, 1x20ml

Store *028899A, *028899B, *028899D and *028899E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.