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Usbio/028903 Alpha-Tocopherol (TCPa) BioAssay™ ELISA Kit/028903/96Tests
Usbio/028903 Alpha-Tocopherol (TCPa) BioAssay™ ELISA Kit/028903/96Tests
商品编号：
028903
品牌：
USbiological
市场价：
¥18760.00
美元价：
7973.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The alpha-Tocopherol (TCPa) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of TCPa in serum, plasma and other biological fluids.
Detection Range
1.23-100ug/ml
Sensitivity
0.52ug/ml
Intra-Assay CV: |<10%
Inter-Assay CV: |<12%
Kit Components
*028903A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated, ready to use*028903B: Standard, 2x1vial 028903C: Standard Diluent, 1x10ml or 1x20ml*028903D: Detection Reagent A (greeen), 1x60ul or 1x120ul *028903E: Detection Reagent B (red), 1x60ul or 1x120ul 028903F: Assay Diluent A, 1x6ml or 1x12ml028903G: Assay Diluent B, 1x6ml or1x12ml028903H: TMB Substrate, 1x4.5ml or 1x9ml*028903J: Positive Control, 54.7ug/ml, 1x1vial (Lyophilized)028903K: Stop Solution, 1x3ml or 1x6ml028903L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution
The Stop Solution (028903K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *02890A, *02890B, *02890D, *02890E and *02890J at -20°C. After reconstitution of *02890J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 028903J: Positive Control:
Intended Use
The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics
TCPa in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.
Expected Values
54.7ug/ml
Usage: |Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
品牌介绍
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