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Usbio/C7510-34A Collagen (Chicken) Type II, Rat Antibody (IgG) BioAssay™ ELISA Kit/C7510-34A/1Kit
Usbio/C7510-34A Collagen (Chicken) Type II, Rat Antibody (IgG) BioAssay™ ELISA Kit/C7510-34A/1Kit
商品编号：
C7510-34A
品牌：
USbiological
市场价：
¥16500.00
美元价：
7012.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
This ELISA kit is designed to assay type II collagen antibodies in rat sera. The microtiter strips provided in the kit have been coated with chicken type II collagen. To reduce non-specific binding, we have incorporated unique blocking agents which inhibit the hydrophobic binding of immunoreactive serum components in sample specimens onto plastic surfaces and thus reduce the background levels.
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched.
Heterologous type II collagen is widely used for inducing arthritis in susceptible animals. The serum antibodies in collagen-induced arthritic (CIA) mice are highly specific to the type II collagen used for immunization, however there is significant crossreactivity with other species of type II collagen including rat type II collagen.
This ELISA kit contains enough material to run two partial plates on two separate occasions (see assay procedure).
Kit Components
C7510-34A1 Standard Antibody, 1x20units. Supplied as lyophilized powder.C7510-34A2 IgG (HRP), 2x50ul. Peroxidase-conjugated goat anti-rat IgG. Supplied as lyophilized powder.C7510-34A3 Blocking Buffer, 1x12mlC7510-34A4 Sample/Standard Dilution buffer, 1x50mlC7510-34A5 IgG (HRP) Dilution Buffer, 1x20mlC7510-34A6 Stop Solution, 1x10ml. 2N sulfuric acid.C7510-34A7 Wash Buffer (20X), 1x50mlC7510-34A8 OPD Dilution Buffer, 1x20mlC7510-34A9 OPD, 2 tabletsC7510-34A10 Microtiter Strips, 10x8-well strips. Coated with chicken collagen type II.C7510-34A11 Reference Standard Strips, 4x8-well strips. Use 2 strips per run.
Storage and Stability
Store all components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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