当前位置：
首页
>
产品中心 >
Sandwich_method_ELISA >
Usbio/384867 Tocilizumab (Actemra®) BioAssay™ ELISA Kit/384867/96Tests
Usbio/384867 Tocilizumab (Actemra®) BioAssay™ ELISA Kit/384867/96Tests
商品编号：
384867
品牌：
USbiological
市场价：
¥22280.00
美元价：
9469.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Tocilizumab (Actemra®) is an immunosuppressive humanized monoclonal antibody drug, mainly for the treatment of rheumatoid arthritis (RA) and systemic juvenile idiopathic arthritis, a severe form of arthritis|in children. Tocilizumab binds the interleukin-6 receptor (IL-6R) thus blocking the signaling caused by interleukin 6 (IL-6). IL-6 is a cytokine that plays an important role in immune response and is implicated in the pathogenesis of many diseases.
Sample Type
Serum and plasma
Intended Use
The Tocilizumab ELISA kit is designed to measure free Tocilizumab with high specificity and sensitivity.
Sensitivity
1.5ng/ml
Recovery
250ng/mL of Tocilizumab was spiked in 10 lots of human serum. Recovery ranges are from 88-112% with an average recovery of 104%.
Precision:t|Intra-assay CV: <10% |Inter-assay CV: <10%.
Specificity
hIgG1, Rituximab, and Infliximab prepared at 250ng/mL were assayed and exhibited no cross-reactivity or interference.
Test Principle
This assay employs the sandwich enzyme immunoassay technique. Anti- Tocilizumab is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Tocilizumab present in biological matrices is bound by the immobilized anti- Tocilizumab antibody. After washing away any unbound substances, enzyme linked anti- Tocilizumab antibody is added to the wells. This antibody is developed and purified specifically against Actemra® (domain residing in Fc portion of the Actemra® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Tocilizumab present in test samples. The color development is stopped and the intensity of the color is measured.
Kit Components
Coated microtiter plate, 1x96 wells Calibrator diluent, 1x1.8mlCalibrator (0.5mg/mL), 1x12ulWash buffer, 10X, 1x25mlAssay buffer 1x50mlDetection reagent, 1000X, 1x17ulTMB, 1x12mlTMB stop solution, 1x12ml
Storage and Stability
Store kit components at -20°C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
恭喜苏州蚂蚁淘获得usbio2020年授权书，成为usbio中国代理，购买usbio,请认准usbio代理-苏州蚂蚁淘！
联络我们
