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Usbio/369979 HSP60 (Heat Shock Protein 60) BioAssay™ ELISA Kit (Bovine)/369979/48Tests
Usbio/369979 HSP60 (Heat Shock Protein 60) BioAssay™ ELISA Kit (Bovine)/369979/48Tests
商品编号：
369979
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Intended Use
Sandwich ELISA, Double Antibody
Sensitivity
<0.188ng/ml
Range
0.313-20ng/ml
Specificity
Bovine
Test Principle
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. HSP60 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HSP60 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HSP60 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HSP60 can be calculated.
Kit Components
Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 120ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 1x30mlPlate Sealer, 5xpiecesProduct Description, 1copy
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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