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Usbio/384868 Trastuzumab (Herceptin®) BioAssay™ ELISA Kit/384868/96Tests
Usbio/384868 Trastuzumab (Herceptin®) BioAssay™ ELISA Kit/384868/96Tests
商品编号：
384868
品牌：
USbiological
市场价：
¥22280.00
美元价：
9469.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Trastuzumab(Herceptin®)isahumanizedmonoclonalantibodyusedinthetreatmentofHER2-positivecancers.TrastuzumabbindstodomainIVoftheextracellulardomainoftheHER2/neureceptor.CellstreatedwithTrastuzumabundergocellcyclearrestduringtheG1phase.IthasbeensuggestedthattrastuzumabdoesnotalterHER-2expression,butdecreasesactivationofAKT.
SampleType
Serumandplasma
IntendedUse
Sensitivity
1.25ng/ml
Recovery
1000ng/mLofTrastuzumabwasspikedin10lotsofhumanserum.Recoveryrangesarefrom91-117%withanaveragerecoveryof106%.
Precision:t|Intra-assayCV:<10%|Inter-assayCV:<10%.
Specificity
hIgG1,Rituximab,andInfliximabpreparedat250ng/mLwereassayedandexhibitednocross-reactivityorinterference.
TestPrinciple
Thisassayemploysthesandwichenzymeimmunoassaytechnique.Anti-Trastuzumabiscoatedontoa96wellmicroplate.Calibrator,qualitycontrolsamples(ifdesired)andtestsamplesarePipettedintotheappropriatewells.Trastuzumabpresentinbiologicalmatricesisboundbytheimmobilizedanti-Trastuzumabantibody.Afterwashingawayanyunboundsubstances,enzymelinkedanti-Trastuzumabantibodyisaddedtothewells.Theplateiswashedtoremoveanyunboundantibody-enzymereagentandasubstratesolutionisaddedtothewellsforcolordevelopment.ThecolordevelopmentisproportionaltotheamountofTrastuzumabpresentintestsamples.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.
KitComponents
Coatedmicrotiterplate,1x96wellsCalibratordiluent,1x1.8mlCalibrator(1mg/mL),1x12ulWashbuffer,10X,1x25mlAssaybuffer1x50mlDetectionreagent,1000X,1x17ulTMB,1x12mlTMBstopsolution,1x12ml
StorageandStABIlity
Storekitcomponentsat-20°Cunlessspecifiedotherwise.Unopenedkitisstablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugesmalltubesonpulsesettingpriortoopening.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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