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Usbio/396648 Epiregulin BioAssay™ ELISA Kit (Porcine)/396648/96Tests
Usbio/396648 Epiregulin BioAssay™ ELISA Kit (Porcine)/396648/96Tests
商品编号：
396648
品牌：
USbiological
市场价：
¥14380.00
美元价：
6111.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminal epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminal may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Sample Type
Cell culture supernates, serum, plasma (heparin or EDTA).
Intended Use
Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Porcine Epiregulin.
Sensitivity
<10pg/ml
Range
156pg/ml-10,000pg/ml
Specificity
Recognizes natural and recombinant Porcine Epiregulin. There is no detectable cross-reactivity with other relevant proteins.
Test Principle
This Porcine Epiregulin BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine Epiregulin has been precoated onto 96-well plates. Standards(E.coli, V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to producea blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine Epiregulin amount of sample captured in plate.
Kit Components
96-well plate precoated with anti-Porcine EREG antibody, 1x plateLyophilized recombinant Porcine EREG standard, 2x10ng/tubeBiotinylated anti-Porcine EREG antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x buffer
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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