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Usbio/396463 PLA2G1B (Phospholipase A2) BioAssay™ ELISA Kit (Mouse)/396463/48Tests
Usbio/396463 PLA2G1B (Phospholipase A2) BioAssay™ ELISA Kit (Mouse)/396463/48Tests
商品编号：
396463
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Intended Use
For quantitative detection of PLA2G1B in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
<0.094ng/ml
Precision: |Intra-Assay CV: <8%|Inter-Assay CV: <10%
Range
0.156-10ng/ml
Specificity
Mouse
Test Principle
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PLA2G1B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PLA2G1B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP- Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PLA2G1B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PLA2G1B can be calculated.
Kit Components
Microtiter Strips, 1x96 wells (8x12 wells)Standard, 2x1 vialSample/Standard Dilution Buffer, 1x20mlAntibody (Biotin) (Concentrated), 1x120ulAntibody Dilution Buffer, 1x10mlStreptavidin (HRP) (SABC), 1x120ulSABC Dilution Buffer, 1x10mlTMB Substrate, 1x10mlStop Solution, 1x10mlWash Buffer, 25X, 1x30ml
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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