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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Usbio/152512 DNA甲基转移酶3A(DNMT3A)生物测定与贸易;ELISA试剂盒(人)/152512/96试验
商品详细Usbio/152512 DNA甲基转移酶3A(DNMT3A)生物测定与贸易;ELISA试剂盒(人)/152512/96试验
Usbio/152512 DNA甲基转移酶3A(DNMT3A)生物测定与贸易;ELISA试剂盒(人)/152512/96试验
Usbio/152512 DNA甲基转移酶3A(DNMT3A)生物测定与贸易;ELISA试剂盒(人)/152512/96试验
商品编号: 152512
品牌: USbiological
市场价: ¥17280.00
美元价: 7344.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

DNAMethyltransferase3A(DNMT3A)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoDNAMethyltransferase3A(DNMT3A).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoDNAMethyltransferase3A(DNMT3A).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainDNAMethyltransferase3A(DNMT3A),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofDNAMethyltransferase3A(DNMT3A)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.

DetectionRange
0.156-10ng/ml
Sensitivity
<0.061ng/ml
SampleMatrix
tissuehomogenates,celllysatesandotherBIOLOGicalfluids.
CrossReactivity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofDNAMethyltransferase3A(DNMT3A).Nosignificantcross-reactivityorinterferencebetweenDNAMethyltransferase3A(DNMT3A)andanalogueswasobserved.
StABIlity
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
StorageandStability
Desiccationrecommended.TheStandard,DetectionReagentA,DetectionReagentBandthe96-wellstripplateshouldbestoredat-20°Cuponreceipt.Storeallotherreagentsat4°C.Stablefor6monthsafterreceiptoforder.
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