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Usbio/152512 DNA甲基转移酶3A(DNMT3A)生物测定与贸易;ELISA试剂盒(人)/152512/96试验
DNAMethyltransferase3A(DNMT3A)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoDNAMethyltransferase3A(DNMT3A).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoDNAMethyltransferase3A(DNMT3A).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainDNAMethyltransferase3A(DNMT3A),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofDNAMethyltransferase3A(DNMT3A)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.