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Usbio/025846 Insulin Like Growth Factor 1 Receptor (IGF1R) BioAssay™ ELISA Kit (Rat)/025846/96Tests
Usbio/025846 Insulin Like Growth Factor 1 Receptor (IGF1R) BioAssay™ ELISA Kit (Rat)/025846/96Tests
商品编号：
025846
品牌：
USbiological
市场价：
¥19020.00
美元价：
8083.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Insulin Like Growth Factor 1 Receptor (IGF1R) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGF1R in rat tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
0.059ng/ml
Intra-Assay CV
<10%
Inter-Assay CV
<12%
Kit Components
*025846A: Microtiter Plate, 1x48 wells or 1x96 wells, Pre-coated; ready to use*025846B: Standard, 2x1 vial 025846C: Standard Diluent, 1x10ml or 1x20ml*025846D: Detection Reagent A (green), 1x60ul or 1x120ul*025846E: Detection Reagent B (red), 1x60ul or 1x120ul025846F: Assay Diluent A, 1x6ml or 1x12ml025846G: Assay Diluent B, 1x6ml or 1x12ml025846H: TMB Substrate, 1x4.5ml or 1x9ml*025846J: Positive Control, 1.15ng/ml, 1x1vial (Lyophilized)025846K: Stop Solution, 1x3ml or 1x6ml025846L: Wash Buffer, 30X, 1x10ml or 1x20ml
Precaution
The Stop Solution (025846K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *025846A, *025846B, *025846D, *025846E and *025846J at -20°C. After reconstitution of *025846J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 025846J: Positive Control:
Intended Use
The control vial is from a rat serum sample known to contain IGF1R. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics
The control vial contains rat IGF1R in rat serum (lyophilized powder). Note: The rat source material used in this product is from healthy rat of SPF standards (specific pathogen-free animals).
Expected Values
1.51ng/ml
Usage
Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.
Assay Procedure Summary
1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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