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Usbio/357836 OVA SIgE (Ovalbumin Specific Immunoglobulin E) BioAssay™ ELISA Kit (Rat)/357836/48Tests
Usbio/357836 OVA SIgE (Ovalbumin Specific Immunoglobulin E) BioAssay™ ELISA Kit (Rat)/357836/48Tests
商品编号：
357836
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheOVAsIgE(OvalbuminSpecificImmunoglobulinE)BioAssay™ELISAKit(Rat)isaquantitativesandwichassayforthedetectionofOVAsIgEinratserum,plasmaandotherBIOLOGicalfluids.
DetectionRange
0.781-50ng/ml
Sensitivity
<0.469ng/ml
Precision:t|Intra-AssayCV:<8%|Inter-AssayCV:<10%
AssayPrinciple
Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoOVAsIgE.Standardsandsamplesareaddedtotheappropriatemicrotiterplatewellsfollowedbyabiotin-conjugatedantibodyspecifictoOVAsIgE.StreptavidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainOVAsIgE,biotin-conjugatedantibodyandenzyme-conjugatedstreptavidincomplexwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulfuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofOVAsIgEinthesampleisthendeterminedbycomparingtheO.D.ofthesampletothestandardcurve.
KitComponents
*357836A:MicrotiterStrips,8x12wells.*357836B:Standard,2vials357836C:Sample/StandardDilutionBuffer,1x20ml357836D:Antibody-Biotin(Concentrated),1x120ul357836E:AntibodyDilutionBuffer,1x10ml*357836F:Streptavidin-HRPConjugate(SABC),1x120ul357836G:SABCDilutionBuffer,1x10ml*357836H:TMBSubstrate,1x10ml357836J:StopSolution,1x10ml357836K:WashBuffer(25X),1x30ml
StorageandStABIlity
Storeunopened*357836Aat4ºC;storeat-20ºConceopened.Storeunopened*357836Bat4°C;oncereconstitutedstoreat4°Cforupto12hoursorat-20°Cforupto48hours.Store*357836Fand*357836Hinthedarkat4°C.Storeallothercomponentsat4°C.Kitisstablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialsafterthawingandpriortoremovingthecap.
AssaySummary
1.Washplate2timesbeforeaddingstandards,samplesandcontrol(zero)towells.2.Add100ulstandardorsampletoeachwellandincubatefor90minutesat37°C3.Aspiratetheliquidandwashplate3times.3.Add100ulAntibody-Biotinworkingsolutiontoeachwellandincubatefor60minutesat37°C4.Aspiratetheliquidandwashplate3times.5.Add100ulSABCworkingsolutiontoeachwell.Incubatefor30minutesat37°C6.Aspiratetheliquidandwashplate5times.7.Add90ulTMBsubstrate.Incubate15-30minutesat37°C8.Add50ulStopSolution.Readat450nmimmediately.9.Calculateresults.
品牌介绍
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