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Usbio/376895 Deoxynivalenol (DON) ELISA BioAssay™ Kit/376895/48Tests
Usbio/376895 Deoxynivalenol (DON) ELISA BioAssay™ Kit/376895/48Tests
商品编号：
376895
品牌：
USbiological
市场价：
¥11960.00
美元价：
5083.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Deoxynivalenol(DON),alsoknownasVomitoxin,belongstotrichotheceneandisproducedbymouldfusarium,growingingrainssuchaswheat,maize,barleyandstover.Becauseofcausingvomittinginporcines,hencethenameisVomitoxin.IthassomeharmfuleffectstohumanbodyandhasbeenlistedasCategoryIIIcarcinogenbyEUclassificationcriteria.In2011,thelimitofDeoxynivalenolresiduesis1000ug/kgingrainsandgrainsproductsinChina.ThisBioAssay™kitisadetectionproductdevelopedbasedonELISAtechnology,withoperationtimeasshortas15minutes.
SampleType
Feed,soybeanmeal
IntendedUse
ThisBioAssay™kitallowsfortheinvitroquantitativedeterminationofDeoxynivalenolconcentrationsinfeed,soybeanmeal.
Sensitivity
1ug/kg
Range
1-81ug/kg
Precision
Intra-assay:CV%<10%Inter-assay:CV%<10%
TestPrinciple
ThisBioAssay™employsthecompetitiveinhibitionenzymeimmunoassaytechnique.ThemicrotiterplateprovidedinthisBioAssay™kithasbeenpre-coatedwithDeoxynivalenolantigen.StandardsorsamplesareaddedtotheappropriatemicrotiterplatewellswithDeoxynivalenolantibodyandHorserADIshPeroxidase(HRP)conjugatedanti-antibody.Thecompetitiveinhibitionreactionislaunchedbetweenpre-coatedDeoxynivalenolandDeoxynivalenolinstandardsorsampleswiththeDeoxynivalenolantibody.AsubstratesolutionisaddedtothewellsandthecolordevelopsinoppositetotheamountofDeoxynivalenolinthestandardsorsamples.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.
KitComponents
Assayplate,96T/48TStandard(96T),6x1mlStandard(48T),6x0.5mlHRP-conjugate(96T),1x7mlHRP-conjugate(48T),1x3.5mlAntibody(96T),1x7mlAntibody(48T),1x1x3.5mlTMBSubstrate(96T),1x12mlTMBSubstrate(48T),1x6mlStopSolution(96T),1x10mlStopSolution,(48T),1x5mlSampleDiluent(96T),1x50mlSampleDIluent(48T),1x25mlWashBuffer(10X)(96T),1x50mlWashBuffer(10X)(48T),1x25mlAdhesiveStrip(96T/48T),4xstripsInstructionManual(96T/48T),1xmanual
StorageandStABIlity
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
品牌介绍
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