Nitrofurans are synthetic broad-spectrum antibiotics, widely used in animal production for its excellent antibacterial and pharmacokinetic properties. They had been also used as growth promoters in the pig production, the poultry and fish sector. In long term studies with experimental animals the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. This has led to a prohibition of Nitrofurans for the treatment of animals used for food production. Current methods of detection Nitrofurazone, such as LC-UV, LC-MS and LC-MS/MS, are with sample preparation complex, expensive, difficult and time-consuming. While using the Nitrofurazone ELISA kit is both rapidly and accurately detect Nitrofurazone residues in samples. This BioAssay™ kit is a detection product developed based on ELISA technology.
Sample Type
Honey, tissue (Chicken, duck meet, porcine, fish, prawn), eggs, etc.
Intended Use
This BioAssay™ kit allows for the in vitro quantitative determination of Nitrofurazone(SEM) concentrations in milk, honey, tissue (fish, shrimp, chicken, porcine, liver).
Precision
Intra-assay: CV%<10%Inter-assay: CV%<10%
Test Principle
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay™ kit has been pre-coated with Nitrofurazone antigen. Standards or samples are added to the appropriate microtiter plate wells with Nitrofurazone specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Nitrofurazone and Nitrofurazone in standards or samples with the Nitrofurazone special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Nitrofurazone in the standards or samples. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate, 96TStandard, 6x1mlHigh Concentration Standard (100ppb), 1x1mlHRP-conjugate, 1x6mlAntibody, 1x6mlSubstrate A, 1x6mlSubstrate B, 1x6mlStop Solution, 1x6mlSample Diluent (4X), 1x40mlWash Buffer (10X), 1x40mlDerivating Reagents (10X), 1x1mlAdhesive Strip (For 96 wells), 4x stripsInstruction Manual, 1x manual
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C.Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
