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Usbio/351016 Platelet-Derived Growth Factor-BB (PDGF-BB), BioAssay™ ELISA Kit (Human)/351016/96Tests
Usbio/351016 Platelet-Derived Growth Factor-BB (PDGF-BB), BioAssay™ ELISA Kit (Human)/351016/96Tests
商品编号：
351016
品牌：
USbiological
市场价：
¥14580.00
美元价：
6196.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Human PDGF (Platelet-Derived Growth Factor) is a general term for a small group of structurally related and secreted growth factors. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation. It is also produced by platelets, macrophages, and mast cells, and at sites of injury, promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new ECM, and IFG-I mediated re-epithelialization.
Sample Type
Serum and plasma
Intended Use
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PDGF-BB in human serum, plasma; and other biological fluids not being tested.
Sensitivity
<1pg/ml
Range
40.9-10,000pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of human PDGF-BB
Precision
Intra CV<10%; Inter CV <15%
Test Principle
This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards, samples and human PDGF-BB mix are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Kit Components
Assay plate (12x8 coated Microwells)-1Standard-2x10uLHuman PDGF-BB Mix-4x9uLAssay diluent (5x concentrate)-1x15mLWash buffer (20x concentrate)-1x25mLTMB substrate-1x12mLStop solution-1x8mLAdhensive strip-2Instruction manual-1
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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