Usbio/152967 Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) BioAssay™ ELISA Kit (Rat)/152967/96Tests-免疫在线蚂蚁淘旗下平台

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商品详细Usbio/152967 Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) BioAssay™ ELISA Kit (Rat)/152967/96Tests

Usbio/152967 Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) BioAssay™ ELISA Kit (Rat)/152967/96Tests

商品编号： 152967

品牌： USbiological

市场价： ¥19400.00

美元价： 8245.00

产地：美国(厂家直采)

公司：

产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

联系Q Q： 3392242852

电话号码： 4000-520-616

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商品介绍

PotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5)BioAssay™ELISAKit(Rat)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.

DetectionRange

0.156-10ng/ml

Sensitivity

<0.055ng/ml

SampleMatrix

TissuehomogenatesandotherBIOLOGicalfluids.

CrossReactivity

ThisassayhashighsensitivityandexcellentspecificityfordetectionofPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5).Nosignificantcross-reactivityorinterferencebetweenPotassiumInwardlyRectifyingChannelSubfamilyJ,Member5(KCNJ5)andanalogueswasobserved.

StABIlity

Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.

StorageandStability

Desiccationrecommended.TheStandard,DetectionReagentA,DetectionReagentBandthe96-wellstripplateshouldbestoredat-20°Cuponreceipt.Storeallotherreagentsat4°C.Stablefor6monthsafterreceiptoforder.

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公司介绍

品牌分类

分子生物学抗体生物化学试剂盒和分析文化媒介生物化学生物制品

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