Usbio/152988 Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) BioAssay™ ELISA Kit (Rat)/152988/96Tests-免疫在线蚂蚁淘旗下平台

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商品详细Usbio/152988 Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) BioAssay™ ELISA Kit (Rat)/152988/96Tests

Usbio/152988 Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) BioAssay™ ELISA Kit (Rat)/152988/96Tests

商品编号： 152988

品牌： USbiological

市场价： ¥18220.00

美元价： 7743.50

产地：美国(厂家直采)

公司：

产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

ProproteinConvertaseSubtilisin/KexinType9(PCSK9)BioAssay™ELISAKit(Rat)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoProproteinConvertaseSubtilisin/KexinType9(PCSK9).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoProproteinConvertaseSubtilisin/KexinType9(PCSK9).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainProproteinConvertaseSubtilisin/KexinType9(PCSK9),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofProproteinConvertaseSubtilisin/KexinType9(PCSK9)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.

DetectionRange

3.125-200ng/ml

Sensitivity

<1.25ng/ml

SampleMatrix

Serum,plasmaandotherBIOLOGicalfluids.

CrossReactivity

ThisassayhashighsensitivityandexcellentspecificityfordetectionofProproteinConvertaseSubtilisin/KexinType9(PCSK9).Nosignificantcross-reactivityorinterferencebetweenProproteinConvertaseSubtilisin/KexinType9(PCSK9)andanalogueswasobserved.

StABIlity

Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.

StorageandStability

Desiccationrecommended.TheStandard,DetectionReagentA,DetectionReagentBandthe96-wellstripplateshouldbestoredat-20°Cuponreceipt.Storeallotherreagentsat4°C.Stablefor6monthsafterreceiptoforder.

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公司介绍

品牌分类

分子生物学抗体生物化学试剂盒和分析文化媒介生物化学生物制品

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