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Usbio/152987 Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) BioAssay™ ELISA Kit (Human)/152987/96Tests
ProproteinConvertaseSubtilisin/KexinType2(PCSK2)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoProproteinConvertaseSubtilisin/KexinType2(PCSK2).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoProproteinConvertaseSubtilisin/KexinType2(PCSK2).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainProproteinConvertaseSubtilisin/KexinType2(PCSK2),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofProproteinConvertaseSubtilisin/KexinType2(PCSK2)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.