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Usbio/152927 Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) BioAssay™ ELISA Kit (Rat)/152927/96Tests
PatatinLikePhospholipaseDomainContainingProtein3(PNPLA3)BioAssay™ELISAKit(Rat)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoPatatinLikePhospholipaseDomainContainingProtein3(PNPLA3).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoPatatinLikePhospholipaseDomainContainingProtein3(PNPLA3).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainPatatinLikePhospholipaseDomainContainingProtein3(PNPLA3),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofPatatinLikePhospholipaseDomainContainingProtein3(PNPLA3)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.
