CitrateSynthase(CS)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoCitrateSynthase(CS).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoCitrateSynthase(CS).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainCitrateSynthase(CS),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofCitrateSynthase(CS)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.
DetectionRange
6.25-400ng/ml
SampleMatrix
Serum,plasma,tissuehomogenatesandother
BIOLOGicalfluids.
CrossReactivity
ThisassayhashighsensitivityandexcellentspecificityfordetectionofCitrateSynthase(CS).Nosignificantcross-reactivityorinterferencebetweenCitrateSynthase(CS)andanalogueswasobserved.
Thestabilityofkitisdeterminedbythelossrateofactivity.Thelossrateofthiskitislessthan5%withintheexpirationdateunderappropriatestoragecondition.Tominimizeextrainfluenceontheperformance,operationproceduresandlabconditions,especiallyroomtemperature,airhumidity,incubatortemperatureshouldbestrictlycontrolled.Itisalsostronglysuggestedthatthewholeassayisperformedbythesameoperatorfromthebeginningtotheend.
StorageandStability
Desiccationrecommended.TheStandard,DetectionReagentA,DetectionReagentBandthe96-wellstripplateshouldbestoredat-20°Cuponreceipt.Storeallotherreagentsat4°C.Stablefor6monthsafterreceiptoforder.
