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Usbio/369746 Anti-TPO (Anti-Thrombopoietin) BioAssay™ ELISA Kit (Human)/369746/48Tests
Usbio/369746 Anti-TPO (Anti-Thrombopoietin) BioAssay™ ELISA Kit (Human)/369746/48Tests
商品编号：
369746
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
SampleType
Serum,plasmaandotherBIOLOGicalfluids.
IntendedUse
SandwichELISA,DoubleAntigen
Sensitivity
<0.938ng/ml
Range
1.563-100ng/ml
Specificity
Human
TestPrinciple
ThisBioAssay™kitwasbasedonsandwichenzyme-linkedimmune-sorbentassaytechnology.TPOwaspre-coatedonto96-wellplates.AndtheBiotin-labeledTPOwasusedasdetectionantigen.Thestandards,testsamplesandBiotin-labeledTPOwereaddedtothewellssubsequently,andwashwithwashbuffer.HRP-StreptavidinConjugatewasaddedandunboundconjugateswerewashedawaywithwashbuffer.TMBsubstrateswereusedtovisualizeHRPenzymaticreaction.TMBwascatalyzedbyHRPtoproduceabluecolorproductthatchangedintoyellowafteraddingacidicstopsolution.Thedensityofyellowisproportionaltotheanti-TPOamountofsamplecapturedinplate.ReadtheO.D.absorbanceat450nminamicroplatereader,andthentheconcentrationofanti-TPOcanbecalculated.
Kit Components
MicroELISAPlate(Dismountable),8x12LyophilizedStandard,2xvialSample/Standarddilutionbuffer,20mlBiotin-detectionantibody(Concentrated),120ulAntibodydilutionbuffer,10mlHRP-StreptavidinConjugate(SABC),120ulSABCdilutionbuffer,10mlTMBsubstrate,10mlStopsolution,10mlWashbuffer(25X),30mlPlateSealer,5xpiecesProductDescription,1xcopy
StorageandStABIlity
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
品牌介绍
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