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Usbio/152312 ATP Binding Cassette Transporter C12 (ABCC12) BioAssay™ ELISA Kit (Human)/152312/96Tests
ATPBindingCassetteTransporterC12(ABCC12)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoATPBindingCassetteTransporterC12(ABCC12).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoATPBindingCassetteTransporterC12(ABCC12).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainATPBindingCassetteTransporterC12(ABCC12),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofATPBindingCassetteTransporterC12(ABCC12)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.