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Usbio/152310 ATP Binding Cassette Transporter C10 (ABCC10) BioAssay™ ELISA Kit (Human)/152310/96Tests
ATPBindingCassetteTransporterC10(ABCC10)BioAssay™ELISAKit(Human)isaSandwichenzymeimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictoATPBindingCassetteTransporterC10(ABCC10).Standardsorsamplesarethenaddedtotheappropriatemicrotiterplatewellswithabiotin-conjugatedantibodyspecifictoATPBindingCassetteTransporterC10(ABCC10).Next,AvidinconjugatedtoHorserADIshPeroxidase(HRP)isaddedtoeachmicroplatewellandincubated.AfterTMBsubstratesolutionisadded,onlythosewellsthatcontainATPBindingCassetteTransporterC10(ABCC10),biotin-conjugatedantibodyandenzyme-conjugatedAvidinwillexhibitachangeincolor.Theenzyme-substratereactionisterminatedbytheadditionofsulphuricacidsolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±10nm.TheconcentrationofATPBindingCassetteTransporterC10(ABCC10)inthesamplesisthendeterminedbycomparingtheO.D.ofthesamplestothestandardcurve.