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Usbio/369740 Anti-Nuclear BioAssay™ ELISA Kit (Human)/369740/48Tests
Usbio/369740 Anti-Nuclear BioAssay™ ELISA Kit (Human)/369740/48Tests
商品编号：
369740
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Sample Type
Serum, plasma and other biological fluids.
Intended Use
Sandwich ELISA, Double Antigen
Sensitivity
<0.938ng/ml
Range
1.563-100ng/ml
Specificity
Human
Test Principle
This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Nuclear was pre-coated onto 96-well plates. And the Biotin-labeled Nuclear was used as detection antigen. The standards, test samples and Biotin-labeled Nuclear were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the anti-nuclear antibody amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of anti-nuclear antibody can be calculated.
Kit Components
Micro ELISA Plate (Dismountable), 8x12Lyophilized Standard, 2xvialSample/Standard dilution buffer, 20mlBiotin-detection antibody (Concentrated), 120ulAntibody dilution buffer, 10mlHRP-Streptavidin Conjugate (SABC), 120ulSABC dilution buffer, 10mlTMB substrate, 10mlStop solution, 10mlWash buffer (25X), 30mlPlate Sealer, 5xpiecesProduct Description, 1xcopy
Storage and Stability
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
品牌介绍
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