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Usbio/361426 VEGF BioAssay™ ELISA Kit (Human) (Vascular Endothelial Growth Factor, VEGF, Vascular Permeability Factor, VPF)/361426/96Tests
Usbio/361426 VEGF BioAssay™ ELISA Kit (Human) (Vascular Endothelial Growth Factor, VEGF, Vascular Permeability Factor, VPF)/361426/96Tests
商品编号：
361426
品牌：
USbiological
市场价：
¥17680.00
美元价：
7514.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
ThegeneforhumanVEGFisorganizedinto8exons.Asaresultofalternativesplicing,atleast4transcriptsencodingmaturemonomericVEGFcontaining121,165,189,and206aaresidues(VEGF121,VEGF165,VEGF189,andVEGF206).VEGFisahighlyconservedproteinthathascross-speciesactivity.Betweenhuman,ratorbovineVEGF,84-94%sequenceidentityhasbeenobserved.Invitro,VEGFisapotentendothelialcellmitogen.Inculturedendothelialcells,VEGFcanactivatephospholipaseCandinducerapidincreasesoffreecytosolicCa2+.VEGFhasalsobeenshowntobechemotacticformonocytesandosteoblasts.Basedonitsinvitroandinvivoproperties,VEGFisexpectedtoplayimportantrolesininflammationandduringnormalandpathologicalangiogenesis,aprocessthatisassociatedwithwoundhealing,embryonicdevelopment,andgrowthandmetastasisofsolidtumors.
SampleType
Cellculturesupernatants,serum,plasma,andtissue
IntendedUse
TheHumanVEGFBioAssay™ELISAKitisanenzyme-linkedimmunosorbentassayforthequantitativedetectionofHumanVEGFconcentrationsincellculturesupernatants,serum,plasma,andtissue.
Sensitivity
4pg/ml
Range
15.625-1000pg/ml
Specificity
RecognizeshumanVEGF.TherewasnodetectablecrossreactivitywithanyofthetestedproteinsfollowingIL-1,2,3,4,5,6,8,10,12,TNF,TGFandIFN.
SampleVolume
100ul/well
TestPrinciple
ThisassayemploystheSandwichimmunoassaytechnique.Ananti-hVEGFmonoclonalcoatingantibodyisadsorbedontomicrowells.VEGFpresentinthesampleorstandardbindstoantibodiesadsorbedtothemicrowells.Followingincubationunboundsampleorstandardareremovedduringawashstep.aBiotinylatedanti-hVEGFantibodyisaddedandbindstoVEGFcapturedbythefirstantibody.FollowingincubationunboundBiotinylatedanti-hVEGFantibodyisremovedduringawashstep.AStreptavidin-HRPisaddedandbindstoBiotinylatedanti-hVEGFantibody.FollowingincubationunboundStreptavidin-HRPisremovedduringawashstep.AcoloredproductisformedinproportiontotheamountofVEGFpresentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsevenVEGFstandarddilutionsandVEGFsampleconcentrationdetermined.
KitComponents
AluminiumpoucheswithaMicrowellPlate:8x12Standard:2xVialBiotinylatedantibody:2xVialStreptavidin-HRP:2xVialAssayBuffer(25ml/Vial):2xVialWashBufferConcentrate20x(30ml/Vial):1xVialTMBSubstrateSolution:1x12mlStopSolution(12ml/Vial):1xVialPlateCovers-Adhesivestrips:3xstripsInstruction:1x
StorageandStABIlity
Storepowderat4°Cliquidat-20°C.Storeothercomponentsat4°C.Stableforatleast6monthsFormaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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