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Usbio/352914 Insulin Like Growth Factor 1 Receptor (IGF1R), BioAssay™ ELISA Kit (Human)/352914/96 Tests
Usbio/352914 Insulin Like Growth Factor 1 Receptor (IGF1R), BioAssay™ ELISA Kit (Human)/352914/96 Tests
商品编号：
352914
品牌：
USbiological
市场价：
¥14980.00
美元价：
6366.50
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IGF1R,alsocalledIGFRorinsulin-likegrowthfactor1receptor,isaproteinthatfoundonthesurfaceofhumancells.Thisgeneismappedto15q26.3.IGF1Rbelongstothelargeclassoftyrosinekinasereceptors.ThisreceptormediatestheeffectsofIGF-1,whichisapolypeptideproteinhormonesimilarinmolecularstructuretoinsulin.Itplaysanimportantroleingrowthandcontinuestohaveanaboliceffectsinadults-meaningthatitcaninducehypertrophyofskeletalmuscleandothertargettissues.IGF1Ralsoplaysacriticalroleintransformationevents.Itishighlyoverexpressedinmostmalignanttissueswhereitfunctionsasananti-apoptoticagentbyenhancingcellsurvival.
IntendedUse
SandwichELISAquantitativedetectionofhumanIGF1Rincellculturesupernates,serumandplasma(heparin,EDTA).
Range
156-10000pg/ml
Sensitivity
<10pg/ml
Specificity
NaturalandrecombinanthumanIGF1R
Crossreactivity
Thereisnodetectablecross-reactivitywithotherrelevantproteins.
TestPrinciple
ThishumanIGF1RBioAssay™ELISAKitwasbasedonstandardsandwichenzyme-linkedimmune-sorbentassaytechnology.AmonoclonalantibodyfrommousespecificforIGF1Rhasbeenprecoatedonto96-wellplates.Standards(NS0,E31-N932)andtestsamplesareaddedtothewells,abiotinylateddetectionpolyclonalantibodyfromgoatspecificforIGF1RisaddedsubsequentlyandthenfollowedbywashingwithPBSorTBSbuffer.Avidin-Biotin-PeroxidaseComplexwasaddedandunboundconjugateswerewashedawaywithPBSorTBSbuffer.HRPsubstrateTMBwasusedtovisualizeHRPenzymaticreaction.TMBwascatalyzedbyHRPtoproduceabluecolorproductthatchangedintoyellowafteraddingacidicstopsolution.ThedensityofyellowisproportionaltothehumanIGF1Ramountofsamplecapturedinplate.
KitComponents
352914A:MicrotiterStrips,1x96wells*352914B:IGF1RStandard,2x10ng352914C:Anti-humanIGF1R(Biotin),1x130ul(1:100dilution)143696:Avidin-Biotin-PeroxidaseComplex(ABC),1x130ul(1:100dilution)143697:SampleDiluentBuffer,1x30ml143698:AntibodyDiluentBuffer,1x12ml143699:ABCDiluentBuffer,1x12ml143700:TMBColorDevelopingAgent,1x10ml143701:TMBStopSolution,2NH2SO4,1x10ml
StorageandStABIlity
Store*352914Bpowderat4°C.Oncereconstitutedstoreat4°Cforupto12hoursorat-20°Cforupto48hours.Storeothercomponentsat4°C.Stablefor6monthsafterreceipt.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoremovingthecap.
AssaySummary
1.Add100ulofsamplesandstandardsandincubatetheplateat37°Cfor90min.Donotwash.2.Add100ulbiotinylatedantibodyandincubatetheplateat37°Cfor60min.Washplate3timeswith0.01MPBSorTBS.3.AddABCworkingsolutionandincubatetheplateat37°Cfor30minutes.Washplate5timeswith0.01MPBSorTBS.4.AddTMBcolordevelopingagentandincubatetheplateat37°Cindarkfor25-30minutes.5.Add100ulTMBstopsolutionandread.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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