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Usbio/023920 Carboxypeptidase A4 (CPA4) BioAssay™ ELISA Kit (Human)/023920/96Tests
Usbio/023920 Carboxypeptidase A4 (CPA4) BioAssay™ ELISA Kit (Human)/023920/96Tests
商品编号：
023920
品牌：
USbiological
市场价：
¥17280.00
美元价：
7344.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Carboxypeptidase A4 (CPA4) BioAssay™ ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CPA4 in human serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
0.065ng/ml
Intra-Assay CV
< 10%
Inter-Assay CV
< 12%
Kit Components
*023920A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*023920B: Standard, 2x1 vial023920C: Standard Diluent, 1x20ml*023920D: Detection Reagent A (green), 1x120ul*023920E: Detection Reagent B (red), 1x120ul023920F: Assay Diluent A, 1x12ml023920G: Assay Diluent B, 1x12ml023920H: TMB Substrate, 1x9ml023920K: Stop Solution, 1x6ml023920L: Wash Buffer, 30X, 1x20ml
Precaution
The Stop Solution (023920K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability
Store *023920A, *023920B, *023920D and *023920E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary
1. Prepare all reagents, samples and standards;2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
品牌介绍
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