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Usbio/369980 HSP70 (Heat Shock Protein 70) BioAssay™ ELISA Kit (Bovine)/369980/48Tests
Usbio/369980 HSP70 (Heat Shock Protein 70) BioAssay™ ELISA Kit (Bovine)/369980/48Tests
商品编号：
369980
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The HSP70 (Heat Shock Protein 70) BioAssay™ ELISA Kit (Bovine) is a quantitative sandwich assay for the detection of HSP70 in bovine serum, plasma, tissue homogenate and other biological fluids.
Detection Range
0.313-20ng/ml
Sensitivity|<0.188ng/ml
Precision: |Intra-Assay CV: <8%|Inter-Assay CV: <10%
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP70 . Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to HSP70 . Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP70 , biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP70 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
369980A: Microtiter Strips, 8x12 wells.369980B: Standard, 2 vials369980C: Sample/Standard Dilution Buffer, 1x20ml369980D: Antibody-Biotin (Concentrated), 1x120ul369980E: Antibody Dilution Buffer, 1x10ml369980F: Streptavidin-HRP Conjugate (SABC), 1x120ul369980G: SABC Dilution Buffer, 1x10m369980H: TMB Substrate, 1x10ml369980J: Stop Solution, 1x10ml369980K: Wash Buffer (25X), 1x30ml
Storage and Stability
Store unopened 369980A at 4ºC; store at -20ºC once opened. Store unopened 369980B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 369980F and 369980H protected from light at 4°C. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary
1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 2 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
品牌介绍
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