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Usbio/354827 Cldn3 (Claudin-3) BioAssay™ ELISA Kit (Mouse)/354827/48Tests
Usbio/354827 Cldn3 (Claudin-3) BioAssay™ ELISA Kit (Mouse)/354827/48Tests
商品编号：
354827
品牌：
USbiological
市场价：
¥11760.00
美元价：
4998.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The Cldn3 (Claudin-3) BioAssay™ ELISA Kit (Mouse) is a quantitative sandwich assay for the detection of Cldn3 in mouse serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Precision: t|Intra-Assay CV: <8%|Inter-Assay CV: <10%
Assay Principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cldn3. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Cldn3. Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cldn3, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cldn3 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
Kit Components
354827A: Microtiter Strips, 8x12 wells.354827B: Standard, 2 vials354827C: Sample/Standard Dilution Buffer, 1x20ml354827D: Antibody-Biotin (Concentrated), 1x120ul354827E: Antibody Dilution Buffer, 1x10ml354827F: Streptavidin-HRP Conjugate (SABC), 1x120ul354827G: SABC Dilution Buffer, 1x10ml354827H: TMB Substrate, 1x10ml354827J: Stop Solution, 1x10ml354827K: Wash Buffer (25X), 1x30ml
Storage and Stability
Store unopened 354827A at 4ºC; store at -20ºC once opened. Store unopened354827B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store 354827F and 354827H protected from light at 4°C. Store all other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Summary
1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 2 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.
品牌介绍
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