Usbio/028370血栓素A2（TXA2）生物测定与贸易；ELISA试剂盒（所有品种）/028370/96试验
商品编号：
028370
品牌：
USbiological
市场价：
¥18960.00
美元价：
8058.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The Thromboxane A2 (TXA2) BioAssay™ ELISA Kit (All Species) is a competitive inhibition immunoassay for the in vitro quantitative measurement of TXA2 in plasma, serum and other biological fluids.
Detection Range
12.35-1,000pg/ml
Sensitivity
5.62pg/ml
Precision
Intra-Assay CV: <10%Inter-Assay CV: <12%
Test Principle:t|This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to TXA2 has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled TXA2 and unlabeled TXA2 in standards or samples for limited binding sites on the pre-coated antibody. After incubation, unbound materials are washed off. Next, avidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of TXA2 in the sample.
Kit Components
*028370: Microtiter Plate, 96 wells, Pre-coated, ready to use.*028370B: Standard, 2x1vial 028370C: Standard Diluent, 1x20ml*028370D: Detection Reagent A, 1x1vial *028370E: Detection Reagent B, 1x120ul 028370F: Assay Diluent A, 1x12ml028370G: Assay Diluent B, 1x12ml028370H: TMB Substrate, 1x9ml028370K: Stop Solution, 1x6mlt028370L: Wash Buffer, 30X, 1x20ml028370M: Reagent Diluent, 1x300ml
Storage and Stability
Store *028370A, *028370B, *028370D and *028370E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Summary of the Assay
1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
品牌介绍
usbio的生化试剂，抗体，重组蛋白，细胞培养基和分子生物学试剂盒几乎用于所有科学应用和环境，包括基因组研究，生物技术，药物开发和疾病诊断。
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